› 3db4

X-ray diffraction
2.4Å resolution

Crystal structure of the tandem tudor domains of the E3 ubiquitin-protein ligase UHRF1

Released:
DOI: 10.2210/pdb3db4/pdb
PDB entry 3db4 coloured by chain, front view.
PDB entry 3db4 coloured by chain, side view.
PDB entry 3db4 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Recognition of multivalent histone states associated with heterochromatin by UHRF1 protein.
Nady N, Lemak A, Walker JR, Avvakumov GV, Kareta MS, Achour M, Xue S, Duan S, Allali-Hassani A, Zuo X, Wang YX, Bronner C, Chédin F, Arrowsmith CH, Dhe-Paganon S
J Biol Chem 286 24300-11 (2011)
PMID: 21489993

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188767 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
E3 ubiquitin-protein ligase UHRF1 Chain: A
Molecule details ›
Chain: A
Length: 161 amino acids
Theoretical weight: 19.01 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q96T88 (Residues: 126-285; Coverage: 20%)
Gene names: ICBP90, NP95, RNF106, UHRF1
Sequence domains: Tandem tudor domain within UHRF1
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P62
Unit cell:
a: 98.654Å b: 98.654Å c: 43.345Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.22 0.217 0.285
Expression system: Escherichia coli

Quick links

Citations

45 review citations

Modification of enhancer chromatin: what, how, and why?
Calo et al. (2013)
44 more

4 mentions without citation

Recognition of multivalent histone states associated with heterochromatin by UHRF1 protein.
Nady et al. (2011)
3 more