› 3d61

X-ray diffraction
1.95Å resolution

Crystal Structure Analysis of 1,5-alpha-arabinanase catalytic mutant (AbnBD147A) complexed to arabinobiose

Released:
DOI: 10.2210/pdb3d61/pdb
PDB entry 3d61 coloured by chain, front view.
PDB entry 3d61 coloured by chain, side view.
PDB entry 3d61 coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Primary publication:
Crystal structure of an inverting GH 43 1,5-alpha-L-arabinanase from Geobacillus stearothermophilus complexed with its substrate.
Alhassid A, Ben-David A, Tabachnikov O, Libster D, Naveh E, Zolotnitsky G, Shoham Y, Shoham G
Biochem J 422 73-82 (2009)
PMID: 19505290

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->5)-alpha-arabinofuranosidic linkages in (1->5)-arabinans
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-109832 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Intracellular endo-alpha-(1->5)-L-arabinanase Chain: A
Molecule details ›
Chain: A
Length: 314 amino acids
Theoretical weight: 35.62 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: B3EYM8 (Residues: 2-315; Coverage: 100%)
Gene name: abnB
Sequence domains: Glycosyl hydrolases family 43
Structure domains: Glycosyl hydrolase domain; family 43

Ligands and Environments

Carbohydrate polymer : NEW Components: FUB, AHR
Carbohydrate polymer
1 bound ligand:
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: C2221
Unit cell:
a: 86.087Å b: 89.732Å c: 75.985Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.185 0.222
Expression system: Escherichia coli

Quick links

Citations

4 review citations

β-xylosidases and α-L-arabinofuranosidases: accessory enzymes for arabinoxylan degradation.
Lagaert et al. (2014)
3 more

1 mention without citation

Broad Analysis of Vicinal Disulfides: Occurrences, Conformations with Cis or with Trans Peptides, and Functional Roles Including Sugar Binding.
Richardson et al. (2017)