› 3d4u

X-ray diffraction
1.7Å resolution

Bovine thrombin-activatable fibrinolysis inhibitor (TAFIa) in complex with tick-derived carboxypeptidase inhibitor.

Released:
DOI: 10.2210/pdb3d4u/pdb
PDB entry 3d4u coloured by chain, front view.
PDB entry 3d4u coloured by chain, side view.
PDB entry 3d4u coloured by chain, top view.
Source organisms:
Primary publication:
Structure of activated thrombin-activatable fibrinolysis inhibitor, a molecular link between coagulation and fibrinolysis.
Sanglas L, Valnickova Z, Arolas JL, Pallarés I, Guevara T, Solà M, Kristensen T, Enghild JJ, Aviles FX, Gomis-Rüth FX
Mol Cell 31 598-606 (2008)
PMID: 18722183

Function and Biology Details

Reaction catalysed: 
Release of C-terminal Arg and Lys from a polypeptide.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173690 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Carboxypeptidase B2 Chain: A
Molecule details ›
Chain: A
Length: 309 amino acids
Theoretical weight: 36.03 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q2KIG3 (Residues: 115-423; Coverage: 77%)
Gene name: CPB2
Sequence domains: Zinc carboxypeptidase
Structure domains: Zn peptidases
Carboxypeptidase inhibitor Chain: B
Molecule details ›
Chain: B
Length: 74 amino acids
Theoretical weight: 7.81 KDa
Source organism: Rhipicephalus bursa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5EPH2 (Residues: 23-96; Coverage: 99%)
Sequence domains: Carboxypeptidase inhibitor I68
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ZN 1 x ZN
Ligand ACT 2 x ACT
Ligand SO4 5 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P3221
Unit cell:
a: 84.2Å b: 84.2Å c: 127.9Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.156 0.156 0.171
Expression system: Escherichia coli

Quick links

Citations

5 review citations

New insights into the molecular mechanisms of the fibrinolytic system.
Rijken et al. (2009)
4 more

3 mentions without citation

Thrombin Activatable Fibrinolysis Inhibitor (TAFI): An Updated Narrative Review.
Sillen et al. (2021)
2 more