› 3cwk

X-ray diffraction
1.6Å resolution

Crystal Structure of the R132K:Y134F:R111L:T54V:L121E Mutant of Cellular Retinoic Acid Binding Protein Type II in Complex with All-trans-Retinoic Acid at 1.57 Angstroms Resolution

Released:
DOI: 10.2210/pdb3cwk/pdb
PDB entry 3cwk coloured by chain, front view.
PDB entry 3cwk coloured by chain, side view.
PDB entry 3cwk coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural analysis of site-directed mutants of cellular retinoic acid-binding protein II addresses the relationship between structural integrity and ligand binding.
Vaezeslami S, Jia X, Vasileiou C, Borhan B, Geiger JH
Acta Crystallogr D Biol Crystallogr 64 1228-39 (2008)
PMID: 19018099

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151430 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cellular retinoic acid-binding protein 2 Chain: A
Molecule details ›
Chain: A
Length: 137 amino acids
Theoretical weight: 15.51 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P29373 (Residues: 2-138; Coverage: 99%)
Gene name: CRABP2
Sequence domains: Lipocalin / cytosolic fatty-acid binding protein family
Structure domains: Lipocalin

Ligands and Environments

2 bound ligands:
Ligand SO4 1 x SO4
Ligand REA 1 x REA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 32-ID
Spacegroup: P212121
Unit cell:
a: 45.787Å b: 44.86Å c: 77.865Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.125 0.12 0.167
Expression system: Escherichia coli

Quick links

Citations

7 citation in other articles

Retinoic acid receptors: from molecular mechanisms to cancer therapy.
di Masi et al. (2015)
6 more

5 mentions without citation

Predicting protein ligand binding sites by combining evolutionary sequence conservation and 3D structure.
Capra et al. (2009)
4 more