› 3ciq

X-ray diffraction
2.9Å resolution

A regulatable switch mediates self-association in an immunoglobulin fold

Released:
DOI: 10.2210/pdb3ciq/pdb
PDB entry 3ciq coloured by chain, front view.
PDB entry 3ciq coloured by chain, side view.
PDB entry 3ciq coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A regulatable switch mediates self-association in an immunoglobulin fold.
Calabrese MF, Eakin CM, Wang JM, Miranker AD
Nat Struct Mol Biol 15 965-71 (2008)
PMID: 19172750

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158381 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-2-microglobulin Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 100 amino acids
Theoretical weight: 11.89 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
Ligand CU 12 x CU
Ligand TLA 1 x TLA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12B
Spacegroup: P1
Unit cell:
a: 67.594Å b: 68.047Å c: 96.218Å
α: 104.38° β: 94.15° γ: 117.77°
R-values:
R R work R free
0.227 0.225 0.264
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Amyloid formation by globular proteins under native conditions.
Chiti et al. (2009)
10 more

13 mentions without citation

Understanding the complex mechanisms of β2-microglobulin amyloid assembly.
Eichner et al. (2011)
12 more