› 3cii

X-ray diffraction
4.41Å resolution

Structure of NKG2A/CD94 bound to HLA-E

Released:
DOI: 10.2210/pdb3cii/pdb
PDB entry 3cii coloured by chain, front view.
PDB entry 3cii coloured by chain, side view.
PDB entry 3cii coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for NKG2A/CD94 recognition of HLA-E.
Kaiser BK, Pizarro JC, Kerns J, Strong RK
Proc Natl Acad Sci U S A 105 6696-701 (2008)
PMID: 18448674

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146744 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Soluble HLA class I histocompatibility antigen, alpha chain E Chains: A, D
Molecule details ›
Chains: A, D
Length: 273 amino acids
Theoretical weight: 31.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P13747 (Residues: 23-295; Coverage: 81%)
Gene names: HLA-6.2, HLA-E, HLAE
Sequence domains:
Beta-2-microglobulin Chains: B, E
Molecule details ›
Chains: B, E
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Soluble HLA class I histocompatibility antigen, alpha chain G Chains: C, F
Molecule details ›
Chains: C, F
Length: 9 amino acids
Theoretical weight: 1.05 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P17693 (Residues: 3-11; Coverage: 0%)
Gene names: HLA-6.0, HLA-G, HLAG
Natural killer cells antigen CD94 Chains: G, I
Molecule details ›
Chains: G, I
Length: 121 amino acids
Theoretical weight: 14.21 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13241 (Residues: 59-179; Coverage: 68%)
Gene names: CD94, KLRD1
Sequence domains: Lectin C-type domain
NKG2-A/NKG2-B type II integral membrane protein Chains: H, J
Molecule details ›
Chains: H, J
Length: 120 amino acids
Theoretical weight: 13.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P26715 (Residues: 113-232; Coverage: 52%)
Gene names: KLRC1, NKG2A
Sequence domains: Lectin C-type domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: I4132
Unit cell:
a: 345.983Å b: 345.983Å c: 345.983Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.321 0.321 0.355
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

53 review citations

NK cells for cancer immunotherapy.
Shimasaki et al. (2020)
52 more

15 mentions without citation

The structural basis of ligand recognition by natural killer cell receptors.
Joyce et al. (2011)
14 more