› 3cfs

X-ray diffraction
2.4Å resolution

Structural basis of the interaction of RbAp46/RbAp48 with histone H4

Released:
DOI: 10.2210/pdb3cfs/pdb
PDB entry 3cfs coloured by chain, front view.
PDB entry 3cfs coloured by chain, side view.
PDB entry 3cfs coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for the recognition of histone H4 by the histone-chaperone RbAp46.
Murzina NV, Pei XY, Zhang W, Sparkes M, Vicente-Garcia J, Pratap JV, McLaughlin SH, Ben-Shahar TR, Verreault A, Luisi BF, Laue ED
Structure 16 1077-85 (2008)
PMID: 18571423

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158679 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Histone-binding protein RBBP7 Chain: B
Molecule details ›
Chain: B
Length: 414 amino acids
Theoretical weight: 46.94 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q16576 (Residues: 1-411; Coverage: 97%)
Gene names: RBAP46, RBBP7
Sequence domains:
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase
Histone H4 Chain: E
Molecule details ›
Chain: E
Length: 15 amino acids
Theoretical weight: 1.7 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P62805 (Residues: 28-42; Coverage: 15%)
Gene names: H4-16, H4/A, H4/B, H4/C, H4/D, H4/E, H4/G, H4/H, H4/I, H4/J, H4/K, H4/M, H4/N, H4/O, H4C1, H4C11, H4C12, H4C13, H4C14, H4C15, H4C16, H4C2, H4C3, H4C4, H4C5, H4C6, H4C8, H4C9, H4F2, H4FA, H4FB, H4FC, H4FD, H4FE, H4FG, H4FH, H4FI, H4FJ, H4FK, H4FM, H4FN, H4FO, HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, HIST1H4E, HIST1H4F, HIST1H4H, HIST1H4I, HIST1H4J, HIST1H4K, HIST1H4L, HIST2H4, HIST2H4A, HIST2H4B, HIST4H4

Ligands and Environments

1 bound ligand:
Ligand ARS 1 x ARS
1 modified residue:
Ligand MSE 3 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 44.665Å b: 85.725Å c: 117.723Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.184 0.249
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli

Quick links

Citations

49 review citations

Epigenetic inheritance during the cell cycle.
Probst et al. (2009)
48 more

14 mentions without citation

The histone shuffle: histone chaperones in an energetic dance.
Das et al. (2010)
13 more