› 3cbl

X-ray diffraction
1.75Å resolution

Crystal structure of human feline sarcoma viral oncogene homologue (v-FES) in complex with staurosporine and a consensus peptide

Released:
DOI: 10.2210/pdb3cbl/pdb
PDB entry 3cbl coloured by chain, front view.
PDB entry 3cbl coloured by chain, side view.
PDB entry 3cbl coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural coupling of SH2-kinase domains links Fes and Abl substrate recognition and kinase activation.
Filippakopoulos P, Kofler M, Hantschel O, Gish GD, Grebien F, Salah E, Neudecker P, Kay LE, Turk BE, Superti-Furga G, Pawson T, Knapp S
Cell 134 793-803 (2008)
PMID: 18775312

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139508 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tyrosine-protein kinase Fes/Fps Chain: A
Molecule details ›
Chain: A
Length: 377 amino acids
Theoretical weight: 42.76 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P07332 (Residues: 448-822; Coverage: 46%)
Gene names: FES, FPS
Sequence domains:
Structure domains:
Synthetic peptide Chain: B
Molecule details ›
Chain: B
Length: 6 amino acids
Theoretical weight: 650 Da
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand STU 3 x STU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: P212121
Unit cell:
a: 35.679Å b: 77.178Å c: 149.49Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.18 0.227
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

31 review citations

Protein kinases: evolution of dynamic regulatory proteins.
Taylor et al. (2011)
30 more

4 mentions without citation

Structural coupling of SH2-kinase domains links Fes and Abl substrate recognition and kinase activation.
Filippakopoulos et al. (2008)
3 more