› 3c7o

X-ray diffraction
1.8Å resolution

Crystal structure of a glycoside hydrolase family 43 arabinoxylan arabinofuranohydrolase from Bacillus subtilis in complex with cellotetraose.

Released:
DOI: 10.2210/pdb3c7o/pdb
PDB entry 3c7o coloured by chain, front view.
PDB entry 3c7o coloured by chain, side view.
PDB entry 3c7o coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Structural analysis of a glycoside hydrolase family 43 arabinoxylan arabinofuranohydrolase in complex with xylotetraose reveals a different binding mechanism compared with other members of the same family.
Vandermarliere E, Bourgois TM, Winn MD, van Campenhout S, Volckaert G, Delcour JA, Strelkov SV, Rabijns A, Courtin CM
Biochem J 418 39-47 (2009)
PMID: 18980579

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175041 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Arabinoxylan arabinofuranohydrolase Chain: A
Molecule details ›
Chain: A
Length: 487 amino acids
Theoretical weight: 51.68 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q45071 (Residues: 27-513; Coverage: 100%)
Gene names: BSU18160, xynD
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC
Carbohydrate polymer
4 bound ligands:
Ligand CA 1 x CA
Ligand NA 2 x NA
Ligand GOL 2 x GOL
Ligand FMT 11 x FMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P212121
Unit cell:
a: 67.99Å b: 73.08Å c: 105.69Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.152 0.151 0.175
Expression system: Escherichia coli

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Citations

8 review citations

The biochemistry and structural biology of plant cell wall deconstruction.
Gilbert HJ. (2010)
7 more