› 3c30

X-ray diffraction
2.8Å resolution

Crystal structure of the Vibrio Cholerae LuxQ periplasmic domain (SeMet)

Released:
DOI: 10.2210/pdb3c30/pdb
PDB entry 3c30 coloured by chain, front view.
PDB entry 3c30 coloured by chain, side view.
PDB entry 3c30 coloured by chain, top view.
Source organism: Vibrio cholerae
Entry authors: Slama B, Hendrickson W

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191950 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Autoinducer 2 sensor kinase/phosphatase LuxQ Chain: A
Molecule details ›
Chain: A
Length: 270 amino acids
Theoretical weight: 30.33 KDa
Source organism: Vibrio cholerae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9KLK7 (Residues: 36-280; Coverage: 29%)
Gene names: VC_A0736, luxQ
Sequence domains: LuxQ, periplasmic
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand MES 1 x MES
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P41212
Unit cell:
a: 55.584Å b: 55.584Å c: 160.959Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.201 0.228
Expression system: Escherichia coli

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