› 3c1m

X-ray diffraction
2.3Å resolution

Cyrstal Structure of threonine-sensitive aspartokinase from Methanococcus jannaschii with MgAMP-PNP and L-aspartate

Released:
DOI: 10.2210/pdb3c1m/pdb
PDB entry 3c1m coloured by chain, front view.
PDB entry 3c1m coloured by chain, side view.
PDB entry 3c1m coloured by chain, top view.
Source organism: Methanocaldococcus jannaschii
Primary publication:
The structural basis for allosteric inhibition of a threonine-sensitive aspartokinase.
Liu X, Pavlovsky AG, Viola RE
J Biol Chem 283 16216-25 (2008)
PMID: 18334478

Function and Biology Details

Reaction catalysed: 
ATP + L-aspartate = ADP + 4-phospho-L-aspartate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176510 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable aspartokinase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 473 amino acids
Theoretical weight: 51.46 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q57991 (Residues: 1-473; Coverage: 100%)
Gene name: MJ0571
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand MG 4 x MG
Ligand ASP 4 x ASP
Ligand ANP 4 x ANP
Ligand FMT 6 x FMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P212121
Unit cell:
a: 101.408Å b: 104.478Å c: 192.045Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.194 0.246
Expression system: Escherichia coli

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Citations

2 review citations

Cohesion group approach for evolutionary analysis of aspartokinase, an enzyme that feeds a branched network of many biochemical pathways.
Lo et al. (2009)
1 more

6 mentions without citation

Prediction of homoprotein and heteroprotein complexes by protein docking and template-based modeling: A CASP-CAPRI experiment.
Lensink et al. (2016)
5 more