› 3bnd

X-ray diffraction
1.6Å resolution

Lipoxygenase-1 (Soybean), I553V Mutant

Released:
DOI: 10.2210/pdb3bnd/pdb
PDB entry 3bnd coloured by chain, front view.
PDB entry 3bnd coloured by chain, side view.
PDB entry 3bnd coloured by chain, top view.
Source organism: Glycine max
Primary publication:
Enzyme structure and dynamics affect hydrogen tunneling: the impact of a remote side chain (I553) in soybean lipoxygenase-1.
Meyer MP, Tomchick DR, Klinman JP
Proc Natl Acad Sci U S A 105 1146-51 (2008)
PMID: 18216254

Function and Biology Details

Reaction catalysed: 
Alpha-linolenate + O(2) = (9Z,11E,13S,15Z)-13-hydroperoxyoctadeca-9,11,15-trienoate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139953 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Seed linoleate 13S-lipoxygenase-1 Chain: A
Molecule details ›
Chain: A
Length: 839 amino acids
Theoretical weight: 94.51 KDa
Source organism: Glycine max
Expression system: Escherichia coli
UniProt:
  • Canonical: P08170 (Residues: 1-839; Coverage: 100%)
Gene names: LOX1, LOX1.1
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand FE 1 x FE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 94.012Å b: 93.004Å c: 49.351Å
α: 90° β: 90.61° γ: 90°
R-values:
R R work R free
0.169 0.168 0.2
Expression system: Escherichia coli

Quick links

Citations

16 review citations

Hydrogen tunneling links protein dynamics to enzyme catalysis.
Klinman et al. (2013)
15 more

1 mention without citation

Impact of Mutations on the Binding Pocket of Soybean Lipoxygenase: Implications for Proton-Coupled Electron Transfer.
Li et al. (2018)