3bdy

X-ray diffraction
2.6Å resolution

Dual specific bH1 Fab in complex with VEGF

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
homo dimer
PDBe Complex ID:
PDB-CPX-213218 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Fab Fragment -Heavy Chain Chain: H
Molecule details ›
Chain: H
Length: 230 amino acids
Theoretical weight: 24.56 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Fab Fragment -Light Chain Chain: L
Molecule details ›
Chain: L
Length: 218 amino acids
Theoretical weight: 23.96 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
N-VEGF Chain: V
Molecule details ›
Chain: V
Length: 102 amino acids
Theoretical weight: 11.95 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P15692 (Residues: 214-315; Coverage: 26%)
Gene names: VEGF, VEGFA
Sequence domains: VEGF heparin-binding domain
Structure domains: Cystine-knot cytokines

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: C2221
Unit cell:
a: 100.6Å b: 197.978Å c: 77.65Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.194 0.252
Expression system: Escherichia coli