› 3bby

X-ray diffraction
1.85Å resolution

Crystal structure of glutathione S-transferase (NP_416804.1) from Escherichia coli K12 at 1.85 A resolution

Released:
DOI: 10.2210/pdb3bby/pdb
PDB entry 3bby coloured by chain, front view.
PDB entry 3bby coloured by chain, side view.
PDB entry 3bby coloured by chain, top view.
Source organism: Escherichia coli K-12
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Reaction catalysed: 
RX + glutathione = HX + R-S-glutathione
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160180 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutathione S-transferase YfcF Chain: A
Molecule details ›
Chain: A
Length: 215 amino acids
Theoretical weight: 24.6 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P77544 (Residues: 1-214; Coverage: 100%)
Gene names: JW2298, b2301, yfcF
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand CA 2 x CA
Ligand ACT 1 x ACT
Ligand ETX 2 x ETX
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P3221
Unit cell:
a: 69.96Å b: 69.96Å c: 92.02Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.161 0.158 0.195
Expression system: Escherichia coli

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