› 3bb1

X-ray diffraction
2.8Å resolution

Crystal structure of Toc34 from Pisum sativum in complex with Mg2+ and GMPPNP

Released:
DOI: 10.2210/pdb3bb1/pdb
PDB entry 3bb1 coloured by chain, front view.
PDB entry 3bb1 coloured by chain, side view.
PDB entry 3bb1 coloured by chain, top view.
Source organism: Pisum sativum
Primary publication:
The GTPase cycle of the chloroplast import receptors Toc33/Toc34: implications from monomeric and dimeric structures.
Koenig P, Oreb M, Höfle A, Kaltofen S, Rippe K, Sinning I, Schleiff E, Tews I
Structure 16 585-96 (2008)
PMID: 18400179

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174941 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Translocase of chloroplast 34 Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 274 amino acids
Theoretical weight: 30.01 KDa
Source organism: Pisum sativum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q41009 (Residues: 1-266; Coverage: 86%)
Gene names: IAP34, OEP34, TOC34
Sequence domains: AIG1 family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

4 bound ligands:
Ligand MG 8 x MG
Ligand GNP 8 x GNP
Ligand PGE 2 x PGE
Ligand GOL 6 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21212
Unit cell:
a: 178.776Å b: 180.061Å c: 90.948Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.227 0.224 0.282
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

14 review citations

Structure-function relationships of the G domain, a canonical switch motif.
Wittinghofer et al. (2011)
13 more

2 mentions without citation

A structural model of a Ras-Raf signalosome.
Mysore et al. (2021)
1 more