3b2e

X-ray diffraction
3Å resolution

Crystal structure of S. cerevisiae Get3 in the open conformation in complex with Get1 cytosolic domain

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-156735 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
ATPase GET3 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 362 amino acids
Theoretical weight: 40.52 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q12154 (Residues: 1-354; Coverage: 100%)
Gene names: ARR4, D2371, GET3, YDL100C
Sequence domains: Anion-transporting ATPase
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Golgi to ER traffic protein 1 Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 84 amino acids
Theoretical weight: 10.06 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P53192 (Residues: 21-104; Coverage: 36%)
Gene names: GET1, MDM39, YGL020C
Sequence domains: CHD5-like protein
Structure domains: Helix hairpin bin

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: C2221
Unit cell:
a: 114.338Å b: 167.663Å c: 244.571Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.274 0.274 0.306
Expression system: Escherichia coli