› 3aut

X-ray diffraction
2Å resolution

Crystal structure of Bacillus megaterium glucose dehydrogenase 4 in complex with NADH

Released:
DOI: 10.2210/pdb3aut/pdb
PDB entry 3aut coloured by chain, front view.
PDB entry 3aut coloured by chain, side view.
PDB entry 3aut coloured by chain, top view.
Source organism: Priestia megaterium
Primary publication:
Structure-guided mutagenesis for the improvement of substrate specificity of Bacillus megaterium glucose 1-dehydrogenase IV.
Nishioka T, Yasutake Y, Nishiya Y, Tamura T
FEBS J 279 3264-75 (2012)
PMID: 22804868

Function and Biology Details

Reaction catalysed: 
D-glucose + NAD(P)(+) = D-glucono-1,5-lactone + NAD(P)H
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153889 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucose 1-dehydrogenase 4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 269 amino acids
Theoretical weight: 29.2 KDa
Source organism: Priestia megaterium
Expression system: Escherichia coli
UniProt:
  • Canonical: P39485 (Residues: 1-261; Coverage: 100%)
Gene name: gdhIV
Sequence domains: Enoyl-(Acyl carrier protein) reductase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments


Cofactor: Ligand NAI 1 x NAI
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NE3A
Spacegroup: C2
Unit cell:
a: 65.017Å b: 128.082Å c: 64.745Å
α: 90° β: 110.44° γ: 90°
R-values:
R R work R free
0.193 0.19 0.241
Expression system: Escherichia coli

Quick links

Citations

2 review citations

From protein engineering to immobilization: promising strategies for the upgrade of industrial enzymes.
Singh et al. (2013)
1 more

2 mentions without citation

3D-Printed Labware for High-Throughput Immobilization of Enzymes.
Spano et al. (2020)
1 more