› 3aib

X-ray diffraction
3.09Å resolution

Crystal Structure of Glucansucrase

Released:
DOI: 10.2210/pdb3aib/pdb
PDB entry 3aib coloured by chain, front view.
PDB entry 3aib coloured by chain, side view.
PDB entry 3aib coloured by chain, top view.
Source organism: Streptococcus mutans
Primary publication:
Crystal structure of glucansucrase from the dental caries pathogen Streptococcus mutans.
Ito K, Ito S, Shimamura T, Weyand S, Kawarasaki Y, Misaka T, Abe K, Kobayashi T, Cameron AD, Iwata S
J Mol Biol 408 177-86 (2011)
PMID: 21354427

Function and Biology Details

Reaction catalysed: 
Sucrose + ((1->6)-alpha-D-glucosyl)(n) = D-fructose + ((1->6)-alpha-D-glucosyl)(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146597 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Glucosyltransferase-SI Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 844 amino acids
Theoretical weight: 94.46 KDa
Source organism: Streptococcus mutans
Expression system: Escherichia coli
UniProt:
  • Canonical: P13470 (Residues: 244-1087; Coverage: 59%)
Gene names: SMU_1005, gtfC
Sequence domains: Glycosyl hydrolase family 70
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
2 bound ligands:
Ligand CA 8 x CA
Ligand MES 8 x MES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NE3A
Spacegroup: P21212
Unit cell:
a: 295.42Å b: 213.94Å c: 220.98Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.211 0.241
Expression system: Escherichia coli

Quick links

Citations

15 review citations

The exopolysaccharide matrix: a virulence determinant of cariogenic biofilm.
Koo et al. (2013)
14 more

7 mentions without citation

Bacterial α-Glucan and Branching Sucrases from GH70 Family: Discovery, Structure-Function Relationship Studies and Engineering.
Molina et al. (2021)
6 more