› 3agf

X-ray diffraction
2.6Å resolution

Crystal structure of Bacillus glutaminase in the presence of 4.3M NaCl

Released:
DOI: 10.2210/pdb3agf/pdb
PDB entry 3agf coloured by chain, front view.
PDB entry 3agf coloured by chain, side view.
PDB entry 3agf coloured by chain, top view.
Source organism: Bacillus subtilis
Entry authors: Yoshimune K, Shirakihara Y, Yumoto I

Function and Biology Details

Reaction catalysed: 
L-glutamine + H(2)O = L-glutamate + NH(3)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128290 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutaminase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 327 amino acids
Theoretical weight: 36.22 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O31465 (Residues: 1-327; Coverage: 100%)
Gene names: BSU02430, glsA, glsA1, ybgJ
Sequence domains: Glutaminase
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: P21212
Unit cell:
a: 70.837Å b: 179.965Å c: 51.878Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.213 0.251
Expression system: Escherichia coli BL21

Quick links