› 3a9e

X-ray diffraction
2.75Å resolution

Crystal structure of a mixed agonist-bound RAR-alpha and antagonist-bound RXR-alpha heterodimer ligand binding domains

Released:
DOI: 10.2210/pdb3a9e/pdb
PDB entry 3a9e coloured by chain, front view.
PDB entry 3a9e coloured by chain, side view.
PDB entry 3a9e coloured by chain, top view.
Source organisms:
Primary publication:
The "Phantom Effect" of the Rexinoid LG100754: structural and functional insights.
Sato Y, Ramalanjaona N, Huet T, Potier N, Osz J, Antony P, Peluso-Iltis C, Poussin-Courmontagne P, Ennifar E, Mély Y, Dejaegere A, Moras D, Rochel N
PLoS One 5 e15119 (2010)
PMID: 21152046

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
hetero hexamer
PDBe Complex ID:
PDB-CPX-145250 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Retinoic acid receptor RXR-alpha Chain: A
Molecule details ›
Chain: A
Length: 240 amino acids
Theoretical weight: 26.77 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P28700 (Residues: 228-467; Coverage: 51%)
Gene names: Nr2b1, Rxra
Sequence domains: Ligand-binding domain of nuclear hormone receptor
Structure domains: Retinoid X Receptor
Retinoic acid receptor alpha Chain: B
Molecule details ›
Chain: B
Length: 269 amino acids
Theoretical weight: 30.5 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P10276 (Residues: 153-421; Coverage: 58%)
Gene names: NR1B1, RARA
Sequence domains: Ligand-binding domain of nuclear hormone receptor
Structure domains: Retinoid X Receptor
Nuclear receptor coactivator 2 Chain: I
Molecule details ›
Chain: I
Length: 13 amino acids
Theoretical weight: 1.58 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q15596 (Residues: 686-698; Coverage: 1%)
Gene names: BHLHE75, NCOA2, SRC2, TIF2

Ligands and Environments

2 bound ligands:
Ligand 754 1 x 754
Ligand REA 1 x REA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P43212
Unit cell:
a: 105.3Å b: 105.3Å c: 111.338Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.201 0.264
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

7 review citations

Non-genomic effects of nuclear receptors: insights from the anucleate platelet.
Unsworth et al. (2018)
6 more

22 mentions without citation

The retinoid X receptors and their ligands.
Dawson et al. (2012)
21 more