› 3a5m

X-ray diffraction
2.4Å resolution

Crystal Structure of a Dictyostelium P109I Mg2+-Actin in Complex with Human Gelsolin Segment 1

Released:
DOI: 10.2210/pdb3a5m/pdb
PDB entry 3a5m coloured by chain, front view.
PDB entry 3a5m coloured by chain, side view.
PDB entry 3a5m coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for actin assembly, activation of ATP hydrolysis, and delayed phosphate release.
Murakami K, Yasunaga T, Noguchi TQ, Gomibuchi Y, Ngo KX, Uyeda TQ, Wakabayashi T
Cell 143 275-87 (2010)
PMID: 20946985

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139011 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Gelsolin Chain: S
Molecule details ›
Chain: S
Length: 127 amino acids
Theoretical weight: 14.21 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P06396 (Residues: 53-176; Coverage: 16%)
Gene name: GSN
Sequence domains: Gelsolin repeat
Structure domains: Severin
Major actin Chain: C
Molecule details ›
Chain: C
Length: 375 amino acids
Theoretical weight: 41.47 KDa
Source organism: Dictyostelium discoideum
Expression system: Dictyostelium
UniProt:
  • Canonical: P07830 (Residues: 2-376; Coverage: 100%)
Gene names: DDB_G0269234, DDB_G0272248, DDB_G0272520, DDB_G0274129, DDB_G0274133, DDB_G0274135, DDB_G0274137, DDB_G0274285, DDB_G0274561, DDB_G0274599, DDB_G0274601, DDB_G0274727, DDB_G0280545, DDB_G0288879, DDB_G0289005, DDB_G0289553, DDB_G0289663, act1, act11, act12, act13, act14, act15, act16, act19, act1a, act2, act2-1, act20, act21, act4, act5, act6, act7, act8, act9, actA1, actA8, actB1, actM6
Sequence domains: Actin
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand CA 2 x CA
Ligand MG 1 x MG
Ligand ATP 1 x ATP
1 modified residue:
Ligand HIC 1 x HIC

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P212121
Unit cell:
a: 56.701Å b: 69.173Å c: 181.69Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.18 0.223
Expression systems:
  • Escherichia coli
  • Dictyostelium

Quick links

Citations

12 review citations

Building distinct actin filament networks in a common cytoplasm.
Michelot et al. (2011)
11 more

1 mention without citation

Structural Significance of His73 in F-Actin Dynamics: Insights from Ab Initio Study.
Li et al. (2022)