Function and Biology Details
Reaction catalysed:
(tRNA(Ile2))-cytidine(34) + L-lysine + ATP = (tRNA(Ile2))-lysidine(34) + AMP + diphosphate + H(2)O
Biochemical function:
Biological process:
Cellular component:
Structure analysis Details
Assembly composition:
hetero dimer (preferred)
Assembly name:
tRNA(Ile)-lysidine synthase and RNA (preferred)
PDBe Complex ID:
PDB-CPX-177430 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
tRNA(Ile)-lysidine synthase
Molecule details ›
Chains: A, B
Length: 464 amino acids
Theoretical weight: 53.19 KDa
Source organism: Geobacillus kaustophilus
Expression system: Escherichia coli
UniProt:
Sequence domains:
Structure domains:
Length: 464 amino acids
Theoretical weight: 53.19 KDa
Source organism: Geobacillus kaustophilus
Expression system: Escherichia coli
UniProt:
- Canonical:
Q5L3T3 (Residues: 1-464; Coverage: 100%)
Sequence domains:
Structure domains:
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
PHOTON FACTORY BEAMLINE AR-NW12A, SPRING-8 BEAMLINE BL41XU
Spacegroup:
P212121
Expression systems:
- Escherichia coli
- Not provided
