› 3a13

X-ray diffraction
2.34Å resolution

Crystal structure of Type III Rubisco SP4 mutant complexed with 2-CABP and activated with Ca

Released:
DOI: 10.2210/pdb3a13/pdb
PDB entry 3a13 coloured by chain, front view.
PDB entry 3a13 coloured by chain, side view.
PDB entry 3a13 coloured by chain, top view.
Source organism: Thermococcus kodakarensis KOD1
Entry authors: Nishitani Y, Fujihashi M, Doi T, Yoshida S, Atomi H, Imanaka T, Miki K

Function and Biology Details

Reaction catalysed: 
2 3-phospho-D-glycerate + 2 H(+) = D-ribulose 1,5-bisphosphate + CO(2) + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo decamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131673 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribulose bisphosphate carboxylase Chains: A, B, C, D, E, F, G, H, I, J
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J
Length: 444 amino acids
Theoretical weight: 49.85 KDa
Source organism: Thermococcus kodakarensis KOD1
Expression system: Escherichia coli
UniProt:
  • Canonical: O93627 (Residues: 1-444; Coverage: 100%)
Gene names: TK2290, rbc, rbcL
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MG 8 x MG
Ligand CAP 10 x CAP
Ligand CA 2 x CA
1 modified residue:
Ligand KCX 10 x KCX

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P21212
Unit cell:
a: 173.678Å b: 247.09Å c: 144.94Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.205 0.25
Expression system: Escherichia coli

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