› 3a05

X-ray diffraction
2.2Å resolution

Crystal structure of tryptophanyl-tRNA synthetase from hyperthermophilic archaeon, Aeropyrum pernix K1 complex with tryptophan

Released:
DOI: 10.2210/pdb3a05/pdb
PDB entry 3a05 coloured by chain, front view.
PDB entry 3a05 coloured by chain, side view.
PDB entry 3a05 coloured by chain, top view.
Source organism: Aeropyrum pernix
Entry authors: Tsuchiya W, Fujimoto Z, Hasegawa T

Function and Biology Details

Reaction catalysed: 
ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195422 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tryptophan--tRNA ligase Chain: A
Molecule details ›
Chain: A
Length: 372 amino acids
Theoretical weight: 42.22 KDa
Source organism: Aeropyrum pernix
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y924 (Residues: 1-372; Coverage: 100%)
Gene names: APE_2461.1, trpS
Sequence domains: tRNA synthetases class I (W and Y)
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand TRP 1 x TRP
Ligand SF4 1 x SF4
Ligand CD 3 x CD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: C2221
Unit cell:
a: 98.212Å b: 102.919Å c: 97.645Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.189 0.244
Expression system: Escherichia coli

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