› 310d

X-ray diffraction
1.3Å resolution

Crystal structure of 2'-O-Me(CGCGCG)2: an RNA duplex at 1.3 A resolution. Hydration pattern of 2'-O-methylated RNA

Released:
DOI: 10.2210/pdb310d/pdb
PDB entry 310d coloured by chain, front view.
PDB entry 310d coloured by chain, side view.
PDB entry 310d coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Crystal structure of 2'-O-Me(CGCGCG)2, an RNA duplex at 1.30 A resolution. Hydration pattern of 2'-O-methylated RNA.
Adamiak DA, Milecki J, Popenda M, Adamiak RW, Dauter Z, Rypniewski WR
Nucleic Acids Res 25 4599-607 (1997)
PMID: 9358171

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-196612 (preferred)
Entry contents:
1 distinct RNA molecule
Macromolecule:
RNA (5'-R(*(OMC)P*(OMG)P*(OMC)P*(OMG)P*(OMC)P*(OMG))-3') Chains: A, B
Molecule details ›
Chains: A, B
Length: 6 nucleotides
Theoretical weight: 1.99 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MG 2 x MG
2 modified residues:
Ligand OMC 6 x OMC
Ligand OMG 6 x OMG

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: P6122
Unit cell:
a: 26.27Å b: 26.27Å c: 160.9Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.172 not available not available
Expression system: Not provided

Quick links

Citations

4 review citations

2'-O-Methylation of Ribosomal RNA: Towards an Epitranscriptomic Control of Translation?
Monaco et al. (2018)
3 more

1 mention without citation

Internal RNA 2'-O-methylation on the HIV-1 genome impairs reverse transcription.
Decombe et al. (2024)