› 2zs1

X-ray diffraction
1.7Å resolution

Structural Basis for the Heterotropic and Homotropic Interactions of Invertebrate Giant Hemoglobin

Released:
DOI: 10.2210/pdb2zs1/pdb
PDB entry 2zs1 coloured by chain, front view.
PDB entry 2zs1 coloured by chain, side view.
PDB entry 2zs1 coloured by chain, top view.
Source organism: Oligobrachia mashikoi
Primary publication:
Structural basis for the heterotropic and homotropic interactions of invertebrate giant hemoglobin.
Numoto N, Nakagawa T, Kita A, Sasayama Y, Fukumori Y, Miki K
Biochemistry 47 11231-8 (2008)
PMID: 18834142

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero 24-mer
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177349 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Extracellular giant hemoglobin major globin subunit A1 Chain: A
Molecule details ›
Chain: A
Length: 140 amino acids
Theoretical weight: 15.19 KDa
Source organism: Oligobrachia mashikoi
UniProt:
  • Canonical: Q7M419 (Residues: 17-156; Coverage: 100%)
Gene name: ghbA1
Sequence domains: Globin
Structure domains: Globins
Extracellular giant hemoglobin major globin subunit A2 Chain: B
Molecule details ›
Chain: B
Length: 142 amino acids
Theoretical weight: 15.33 KDa
Source organism: Oligobrachia mashikoi
UniProt:
  • Canonical: Q7M413 (Residues: 17-158; Coverage: 100%)
Gene name: ghbA2
Sequence domains: Globin
Structure domains: Globins
Extracellular giant hemoglobin major globin subunit B2 Chain: C
Molecule details ›
Chain: C
Length: 147 amino acids
Theoretical weight: 15.62 KDa
Source organism: Oligobrachia mashikoi
UniProt:
  • Canonical: Q7M418 (Residues: 17-163; Coverage: 100%)
Gene name: ghbB2
Sequence domains: Globin
Structure domains: Globins
Extracellular giant hemoglobin major globin subunit B1 Chain: D
Molecule details ›
Chain: D
Length: 145 amino acids
Theoretical weight: 14.69 KDa
Source organism: Oligobrachia mashikoi
UniProt:
  • Canonical: Q5KSB7 (Residues: 17-161; Coverage: 100%)
Gene name: ghbB1
Sequence domains: Globin
Structure domains: Globins

Ligands and Environments

5 bound ligands:
Ligand HEM 4 x HEM
Ligand OXY 4 x OXY
Ligand GOL 6 x GOL
Ligand CL 1 x CL
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: R32
Unit cell:
a: 111.302Å b: 111.302Å c: 274.93Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.18 0.18 0.207

Quick links

Citations

1 review citation

Tertiary and quaternary effects in the allosteric regulation of animal hemoglobins.
Ronda et al. (2013)
7 other articles

1 mention without citation

Conservation of the three-dimensional structure in non-homologous or unrelated proteins.
Sousounis et al. (2012)