› 2zpc

X-ray diffraction
2.35Å resolution

Crystal structure of the R43L mutant of LolA in the closed form

Released:
DOI: 10.2210/pdb2zpc/pdb
PDB entry 2zpc coloured by chain, front view.
PDB entry 2zpc coloured by chain, side view.
PDB entry 2zpc coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Opening and closing of the hydrophobic cavity of LolA coupled to lipoprotein binding and release.
Oguchi Y, Takeda K, Watanabe S, Yokota N, Miki K, Tokuda H
J Biol Chem 283 25414-25420 (2008)
PMID: 18617521

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158251 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Outer-membrane lipoprotein carrier protein Chain: A
Molecule details ›
Chain: A
Length: 190 amino acids
Theoretical weight: 21.37 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P61316 (Residues: 22-203; Coverage: 100%)
Gene names: JW0874, b0891, lolA, lplA, yzzV
Sequence domains: Outer membrane lipoprotein carrier protein LolA
Structure domains: Lipoprotein localisation LolA/LolB/LppX

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44B2
Spacegroup: P3221
Unit cell:
a: 60.982Å b: 60.982Å c: 78.754Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.241 0.241 0.275
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Lipoprotein sorting in bacteria.
Okuda et al. (2011)
2 more

1 mention without citation

LolA and LolB from the plant-pathogen Xanthomonas campestris forms a stable heterodimeric complex in the absence of lipoprotein.
Furlanetto et al. (2023)