› 2zoz

X-ray diffraction
1.95Å resolution

Crystal structure of the ethidium-bound form of the multi-drug binding transcriptional repressor CgmR

Released:
DOI: 10.2210/pdb2zoz/pdb
PDB entry 2zoz coloured by chain, front view.
PDB entry 2zoz coloured by chain, side view.
PDB entry 2zoz coloured by chain, top view.
Source organism: Corynebacterium glutamicum ATCC 13032
Primary publication:
Crystal structures of the multidrug binding repressor Corynebacteriumglutamicum CgmR in complex with inducers and with an operator.
Itou H, Watanabe N, Yao M, Shirakihara Y, Tanaka I
J Mol Biol 403 174-84 (2010)
PMID: 20691702

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185602 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH tetR-type domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 183 amino acids
Theoretical weight: 21.13 KDa
Source organism: Corynebacterium glutamicum ATCC 13032
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8NMG3 (Residues: 1-175; Coverage: 99%)
Gene name: Cgl2612
Sequence domains:
Structure domains: Tetracycline Repressor, domain 2

Ligands and Environments

3 bound ligands:
Ligand SO4 3 x SO4
Ligand GOL 6 x GOL
Ligand ET 1 x ET
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: P212121
Unit cell:
a: 62.745Å b: 92.226Å c: 105.684Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.207 0.235
Expression system: Escherichia coli

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Citations

6 review citations

Transcription factor-based biosensors enlightened by the analyte.
Fernandez-López et al. (2015)
5 more

3 mentions without citation

The TetR family of regulators.
Cuthbertson et al. (2013)
2 more