› 2zat

X-ray diffraction
1.5Å resolution

Crystal structure of a mammalian reductase

Released:
DOI: 10.2210/pdb2zat/pdb
PDB entry 2zat coloured by chain, front view.
PDB entry 2zat coloured by chain, side view.
PDB entry 2zat coloured by chain, top view.
Source organism: Sus scrofa
Primary publication:
Molecular basis for peroxisomal localization of tetrameric carbonyl reductase.
Tanaka N, Aoki K, Ishikura S, Nagano M, Imamura Y, Hara A, Nakamura KT
Structure 16 388-97 (2008)
PMID: 18334214

Function and Biology Details

Reactions catalysed: 
R-CHOH-R' + NADP(+) = R-CO-R' + NADPH
All-trans-retinol + NADP(+) = all-trans-retinal + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186672 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dehydrogenase/reductase SDR family member 4 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 260 amino acids
Theoretical weight: 27.81 KDa
Source organism: Sus scrofa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8WNV7 (Residues: 20-279; Coverage: 93%)
Gene name: DHRS4
Sequence domains: Enoyl-(Acyl carrier protein) reductase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments


Cofactor: Ligand NAP 4 x NAP
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P42
Unit cell:
a: 109.612Å b: 109.612Å c: 94.308Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.162 0.161 0.187
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Multiple pathways for protein transport to peroxisomes.
Kim et al. (2015)
5 more

2 mentions without citation

In silico identification of epitopes in Mycobacterium avium subsp. paratuberculosis proteins that were upregulated under stress conditions.
Gurung et al. (2012)
1 more