› 2z3t

X-ray diffraction
1.9Å resolution

Crystal Structure of Substrate Free Cytochrome P450 StaP (CYP245A1)

Released:
DOI: 10.2210/pdb2z3t/pdb
PDB entry 2z3t coloured by chain, front view.
PDB entry 2z3t coloured by chain, side view.
PDB entry 2z3t coloured by chain, top view.
Source organism: Streptomyces sp. TP-A0274
Primary publication:
Crystal structures and catalytic mechanism of cytochrome P450 StaP that produces the indolocarbazole skeleton.
Makino M, Sugimoto H, Shiro Y, Asamizu S, Onaka H, Nagano S
Proc Natl Acad Sci U S A 104 11591-6 (2007)
PMID: 17606921

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182974 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome P450 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 425 amino acids
Theoretical weight: 47.26 KDa
Source organism: Streptomyces sp. TP-A0274
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q83WG3 (Residues: 1-417; Coverage: 100%)
Gene name: staP
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments

3 bound ligands:
Ligand HEM 4 x HEM
Ligand IMD 4 x IMD
Ligand EDO 9 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44B2
Spacegroup: P21
Unit cell:
a: 89.64Å b: 78.718Å c: 145.656Å
α: 90° β: 107.92° γ: 90°
R-values:
R R work R free
0.228 0.228 0.273
Expression system: Escherichia coli BL21(DE3)

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Citations

17 review citations

Diversity of P450 enzymes in the biosynthesis of natural products.
Podust et al. (2012)
16 more

5 mentions without citation

Cytochromes P450 for natural product biosynthesis in Streptomyces: sequence, structure, and function.
Rudolf et al. (2017)
4 more