› 2ywr

X-ray diffraction
1.77Å resolution

Crystal structure of GAR transformylase from Aquifex aeolicus

Released:
DOI: 10.2210/pdb2ywr/pdb
PDB entry 2ywr coloured by chain, front view.
PDB entry 2ywr coloured by chain, side view.
PDB entry 2ywr coloured by chain, top view.
Source organism: Aquifex aeolicus
Primary publication:
Structures and reaction mechanisms of the two related enzymes, PurN and PurU.
Sampei G, Kanagawa M, Baba S, Shimasaki T, Taka H, Mitsui S, Fujiwara S, Yanagida Y, Kusano M, Suzuki S, Terao K, Kawai H, Fukai Y, Nakagawa N, Ebihara A, Kuramitsu S, Yokoyama S, Kawai G
J Biochem 154 569-79 (2013)
PMID: 24108189

Function and Biology Details

Reaction catalysed: 
10-formyltetrahydrofolate + N(1)-(5-phospho-D-ribosyl)glycinamide = tetrahydrofolate + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-130409 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoribosylglycinamide formyltransferase Chain: A
Molecule details ›
Chain: A
Length: 216 amino acids
Theoretical weight: 24.32 KDa
Source organism: Aquifex aeolicus
Expression system: Escherichia coli
UniProt:
  • Canonical: O67023 (Residues: 1-216; Coverage: 100%)
Gene names: aq_857, purN
Sequence domains: Formyl transferase
Structure domains: Formyl transferase, N-terminal domain

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand CO 4 x CO
1 modified residue:
Ligand MSE 3 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: P41212
Unit cell:
a: 59.955Å b: 59.955Å c: 105.934Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.204 0.219
Expression system: Escherichia coli

Quick links

Citations

3 citation in other articles

The In Vitro Redundant Enzymes PurN and PurT Are Both Essential for Systemic Infection of Mice in Salmonella enterica Serovar Typhimurium.
Jelsbak et al. (2016)
2 more

1 mention without citation

Expression, crystallization and preliminary X-ray analysis of the phosphoribosylglycinamide formyltransferase from Streptococcus mutans.
Zhai et al. (2011)