› 2yqu

X-ray diffraction
1.7Å resolution

Crystal structures and evolutionary relationship of two different lipoamide dehydrogenase(E3s) from Thermus thermophilus

Released:
DOI: 10.2210/pdb2yqu/pdb
PDB entry 2yqu coloured by chain, front view.
PDB entry 2yqu coloured by chain, side view.
PDB entry 2yqu coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Entry authors: Kondo H, Hossain MT, Adachi W, Nakai T, Kamiya N, Kuramitsu K

Function and Biology Details

Reaction catalysed: 
Protein N(6)-(dihydrolipoyl)lysine + NAD(+) = protein N(6)-(lipoyl)lysine + NADH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178176 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dihydrolipoyl dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 455 amino acids
Theoretical weight: 49.13 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5SLK6 (Residues: 1-455; Coverage: 100%)
Gene name: TTHA0287
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 2 x FAD
2 bound ligands:
Ligand CO3 3 x CO3
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P21
Unit cell:
a: 54.411Å b: 105.76Å c: 84.907Å
α: 90° β: 92.65° γ: 90°
R-values:
R R work R free
0.199 0.195 0.23
Expression system: Escherichia coli BL21(DE3)

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