› 2ygk

X-ray diffraction
2.5Å resolution

Crystal structure of the NurA nuclease from Sulfolobus solfataricus

Released:
DOI: 10.2210/pdb2ygk/pdb
PDB entry 2ygk coloured by chain, front view.
PDB entry 2ygk coloured by chain, side view.
PDB entry 2ygk coloured by chain, top view.
Source organism: Saccharolobus solfataricus
Primary publication:
Structural and functional insights into DNA-end processing by the archaeal HerA helicase-NurA nuclease complex.
Blackwood JK, Rzechorzek NJ, Abrams AS, Maman JD, Pellegrini L, Robinson NP
Nucleic Acids Res 40 3183-96 (2012)
PMID: 22135300

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189014 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA double-strand break repair nuclease NurA Chains: A, B
Molecule details ›
Chains: A, B
Length: 339 amino acids
Theoretical weight: 39.65 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q97WH1 (Residues: 1-339; Coverage: 100%)
Gene names: SSO2248, nurA
Sequence domains: NurA domain

Ligands and Environments

1 bound ligand:
Ligand MN 2 x MN
1 modified residue:
Ligand MSE 20 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P21
Unit cell:
a: 67.74Å b: 80.28Å c: 67.67Å
α: 90° β: 107.17° γ: 90°
R-values:
R R work R free
0.234 0.232 0.271
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

End resection at double-strand breaks: mechanism and regulation.
Symington LS. (2014)
6 more

8 mentions without citation

Structural studies of DNA end detection and resection in homologous recombination.
Schiller et al. (2014)
7 more