› 2yg2

X-ray diffraction
1.7Å resolution

Structure of apolioprotein M in complex with Sphingosine 1-Phosphate

Released:
DOI: 10.2210/pdb2yg2/pdb
PDB entry 2yg2 coloured by chain, front view.
PDB entry 2yg2 coloured by chain, side view.
PDB entry 2yg2 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Endothelium-protective sphingosine-1-phosphate provided by HDL-associated apolipoprotein M.
Christoffersen C, Obinata H, Kumaraswamy SB, Galvani S, Ahnström J, Sevvana M, Egerer-Sieber C, Muller YA, Hla T, Nielsen LB, Dahlbäck B
Proc Natl Acad Sci U S A 108 9613-8 (2011)
PMID: 21606363

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131932 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Apolipoprotein M Chains: A, B
Molecule details ›
Chains: A, B
Length: 172 amino acids
Theoretical weight: 19.24 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O95445 (Residues: 22-188; Coverage: 100%)
Gene names: APOM, G3A, HSPC336, NG20
Sequence domains: ApoM domain
Structure domains: Lipocalin

Ligands and Environments

2 bound ligands:
Ligand S1P 2 x S1P
Ligand FLC 1 x FLC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P3121
Unit cell:
a: 68.28Å b: 68.28Å c: 135.58Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.192 0.191 0.221
Expression system: Escherichia coli

Quick links

Citations

154 review citations

Sphingolipid metabolites in inflammatory disease.
Maceyka et al. (2014)
153 more

7 mentions without citation

'Crystal' Clear? Lysophospholipid Receptor Structure Insights and Controversies.
Blaho et al. (2018)
6 more