› 2yab

X-ray diffraction
1.9Å resolution

Crystal structure of the autoinhibited form of mouse DAPK2 in complex with AMP

Released:
DOI: 10.2210/pdb2yab/pdb
PDB entry 2yab coloured by chain, front view.
PDB entry 2yab coloured by chain, side view.
PDB entry 2yab coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structure of the dimeric autoinhibited conformation of DAPK2, a pro-apoptotic protein kinase.
Patel AK, Yadav RP, Majava V, Kursula I, Kursula P
J Mol Biol 409 369-83 (2011)
PMID: 21497605

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186562 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Death-associated protein kinase 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 361 amino acids
Theoretical weight: 41.8 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8VDF3 (Residues: 11-370; Coverage: 97%)
Gene name: Dapk2
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand AMP 2 x AMP
Ligand SO4 5 x SO4
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X12
Spacegroup: P212121
Unit cell:
a: 63.14Å b: 85.37Å c: 123.99Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.172 0.215
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

The DAPK family: a structure-function analysis.
Shiloh et al. (2014)
6 more

1 mention without citation

Automated refinement of macromolecular structures at low resolution using prior information.
Kovalevskiy et al. (2016)