› 2y6n

X-ray diffraction
1.26Å resolution

STRUCTURE OF LINEAR GRAMICIDIN D OBTAINED USING TYPE I CRYSTALS GROWN IN A 8.8 MONOACYLGLYCEROL LIPID CUBIC PHASE.

Released:
DOI: 10.2210/pdb2y6n/pdb
PDB entry 2y6n coloured by chain, front view.
PDB entry 2y6n coloured by chain, side view.
PDB entry 2y6n coloured by chain, top view.
Source organism: Brevibacillus brevis
Primary publication:
Membrane Protein Crystallization in Lipidic Mesophases. Hosting lipid affects on the crystallization and structure of a transmembrane peptide.
Höfer N, Aragão D, Lyons JA, Caffrey M
Cryst Growth Des 11 1182-1192 (2011)
PMID: 22933857

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-163354 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
VAL-GRAMICIDIN A Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 16 amino acids
Theoretical weight: 1.88 KDa
Source organism: Brevibacillus brevis

Ligands and Environments

2 bound ligands:
Ligand 15P 3 x 15P
Ligand P4C 1 x P4C
3 modified residues:
Ligand FVA 6 x FVA
Ligand DLE 24 x DLE
Ligand DVA 12 x DVA

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P21
Unit cell:
a: 30.616Å b: 62.783Å c: 30.602Å
α: 90° β: 100.02° γ: 90°
R-values:
R R work R free
0.149 0.148 0.17

Quick links

Citations

6 citation in other articles

Structural insights into electron transfer in caa3-type cytochrome oxidase.
Lyons et al. (2012)
5 more

2 mentions without citation

A comprehensive review of the lipid cubic phase or in meso method for crystallizing membrane and soluble proteins and complexes.
Caffrey M. (2015)
1 more