› 2y6m

X-ray diffraction
1.7Å resolution

Crystal structure of EphA4 kinase domain

Released:
DOI: 10.2210/pdb2y6m/pdb
PDB entry 2y6m coloured by chain, front view.
PDB entry 2y6m coloured by chain, side view.
PDB entry 2y6m coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Crystal structure of the EphA4 protein tyrosine kinase domain in the apo- and dasatinib-bound state.
Farenc C, Celie PH, Tensen CP, de Esch IJ, Siegal G
FEBS Lett 585 3593-9 (2011)
PMID: 22036717

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169846 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ephrin type-A receptor 4 Chain: A
Molecule details ›
Chain: A
Length: 291 amino acids
Theoretical weight: 32.93 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q03137 (Residues: 606-896; Coverage: 30%)
Gene names: Epha4, Sek, Sek1
Sequence domains: Protein tyrosine and serine/threonine kinase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 32.513Å b: 91.045Å c: 97.738Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.175 0.203
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Therapeutic targeting of EPH receptors and their ligands.
Boyd et al. (2014)
1 more

2 mentions without citation

Direct Phosphorylation of SRC Homology 3 Domains by Tyrosine Kinase Receptors Disassembles Ligand-Induced Signaling Networks.
Dionne et al. (2018)
1 more