› 2y5y

X-ray diffraction
3.38Å resolution

Crystal structure of LacY in complex with an affinity inactivator

Released:
DOI: 10.2210/pdb2y5y/pdb
PDB entry 2y5y coloured by chain, front view.
PDB entry 2y5y coloured by chain, side view.
PDB entry 2y5y coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Crystal structure of lactose permease in complex with an affinity inactivator yields unique insight into sugar recognition.
Chaptal V, Kwon S, Sawaya MR, Guan L, Kaback HR, Abramson J
Proc Natl Acad Sci U S A 108 9361-6 (2011)
PMID: 21593407

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136384 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lactose permease Chains: A, B
Molecule details ›
Chains: A, B
Length: 423 amino acids
Theoretical weight: 47.27 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P02920 (Residues: 1-417; Coverage: 100%)
Gene names: JW0334, b0343, lacY
Sequence domains: LacY proton/sugar symporter
Structure domains: MFS general substrate transporter like domains

Ligands and Environments

2 bound ligands:
Ligand BA 2 x BA
Ligand TGA 2 x TGA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P212121
Unit cell:
a: 102.56Å b: 127.84Å c: 189.87Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.287 0.286 0.303
Expression system: Escherichia coli K-12

Quick links

Citations

9 review citations

Insights into Protein-Ligand Interactions: Mechanisms, Models, and Methods.
Du et al. (2016)
8 more

10 mentions without citation

Characterization of Lipid-Protein Interactions and Lipid-Mediated Modulation of Membrane Protein Function through Molecular Simulation.
Muller et al. (2019)
9 more