› 2xu0

X-ray diffraction
2.06Å resolution

Crystal structure of the NTS-DBL1(alpha-1) domain of the Plasmodium falciparum membrane protein 1 (PfEMP1) from the varO strain.

Released:
DOI: 10.2210/pdb2xu0/pdb
PDB entry 2xu0 coloured by chain, front view.
PDB entry 2xu0 coloured by chain, side view.
PDB entry 2xu0 coloured by chain, top view.
Source organism: Plasmodium falciparum Palo Alto/Uganda
Primary publication:
Structure of a Plasmodium falciparum PfEMP1 rosetting domain reveals a role for the N-terminal segment in heparin-mediated rosette inhibition.
Juillerat A, Lewit-Bentley A, Guillotte M, Gangnard S, Hessel A, Baron B, Vigan-Womas I, England P, Mercereau-Puijalon O, Bentley GA
Proc Natl Acad Sci U S A 108 5243-8 (2011)
PMID: 21402930

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110601 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Erythrocyte membrane protein 1 Chain: A
Molecule details ›
Chain: A
Length: 487 amino acids
Theoretical weight: 56.41 KDa
Source organism: Plasmodium falciparum Palo Alto/Uganda
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: B7T1P0 (Residues: 2-487; Coverage: 20%)
Gene name: varO
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand PRO 1 x PRO
Ligand MG 1 x MG
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1, ESRF BEAMLINE ID23-1
Spacegroup: P41212
Unit cell:
a: 93.248Å b: 93.248Å c: 126.98Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.182 0.216
Expression system: Escherichia coli K-12

Quick links

Citations

8 review citations

Immunopathogenesis of falciparum malaria: implications for adjunctive therapy in the management of severe and cerebral malaria.
Higgins et al. (2011)
7 more

11 mentions without citation

Plasmodium falciparum Blood Stage Antimalarial Vaccines: An Analysis of Ongoing Clinical Trials and New Perspectives Related to Synthetic Vaccines.
Salamanca et al. (2019)
10 more