› 2xqa

X-ray diffraction
3.7Å resolution

Pentameric ligand gated ion channel GLIC in complex with tetrabutylantimony (TBSb)

Released:
DOI: 10.2210/pdb2xqa/pdb
PDB entry 2xqa coloured by chain, front view.
PDB entry 2xqa coloured by chain, side view.
PDB entry 2xqa coloured by chain, top view.
Source organism: Gloeobacter violaceus PCC 7421
Primary publication:
Structural basis of open channel block in a prokaryotic pentameric ligand-gated ion channel.
Hilf RJ, Bertozzi C, Zimmermann I, Reiter A, Trauner D, Dutzler R
Nat Struct Mol Biol 17 1330-6 (2010)
PMID: 21037567

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181806 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Proton-gated ion channel Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 317 amino acids
Theoretical weight: 36.28 KDa
Source organism: Gloeobacter violaceus PCC 7421
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7NDN8 (Residues: 43-359; Coverage: 100%)
Gene names: glr4197, glvI
Sequence domains: Neurotransmitter-gated ion-channel ligand binding domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SB 1 x SB
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: C2
Unit cell:
a: 183.314Å b: 128.31Å c: 164.374Å
α: 90° β: 104.04° γ: 90°
R-values:
R R work R free
0.257 0.256 0.274
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

12 review citations

Allostery and the Monod-Wyman-Changeux model after 50 years.
Changeux JP. (2012)
11 more

1 mention without citation

X-Ray Crystallographic Studies for Revealing Binding Sites of General Anesthetics in Pentameric Ligand-Gated Ion Channels.
Chen et al. (2018)