› 2xlu

X-ray diffraction
2.6Å resolution

Joint-functions of protein residues and NADP(H) in oxygen-activation by flavin-containing monooxygenase: complex with thioNADP

Released:
DOI: 10.2210/pdb2xlu/pdb
PDB entry 2xlu coloured by chain, front view.
PDB entry 2xlu coloured by chain, side view.
PDB entry 2xlu coloured by chain, top view.
Source organism: Methylophaga aminisulfidivorans
Primary publication:
Joint functions of protein residues and NADP(H) in oxygen activation by flavin-containing monooxygenase.
Orru R, Pazmiño DE, Fraaije MW, Mattevi A
J Biol Chem 285 35021-8 (2010)
PMID: 20807767

Function and Biology Details

Reaction catalysed: 
N,N,N-trimethylamine + NADPH + O(2) = N,N,N-trimethylamine N-oxide + NADP(+) + H(2)O
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182978 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Trimethylamine monooxygenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 461 amino acids
Theoretical weight: 53.34 KDa
Source organism: Methylophaga aminisulfidivorans
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q83XK4 (Residues: 1-456; Coverage: 100%)
Sequence domains: Flavin-binding monooxygenase-like
Structure domains: FAD/NAD(P)-binding domain

Ligands and Environments


Cofactor: Ligand FAD 4 x FAD
3 bound ligands:
Ligand NA7 4 x NA7
Ligand EPE 2 x EPE
Ligand PEG 6 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P61
Unit cell:
a: 220.679Å b: 220.679Å c: 130.531Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.212 0.21 0.252
Expression system: Escherichia coli BL21(DE3)

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Citations

3 review citations

Heteroatom-Heteroatom Bond Formation in Natural Product Biosynthesis.
Waldman et al. (2017)
2 more

1 mention without citation

Joint functions of protein residues and NADP(H) in oxygen activation by flavin-containing monooxygenase.
Orru et al. (2010)