› 2xic

X-ray diffraction
2.9Å resolution

Pilus-presented adhesin, Spy0125 (Cpa), P212121 form (ESRF data)

Released:
DOI: 10.2210/pdb2xic/pdb
PDB entry 2xic coloured by chain, front view.
PDB entry 2xic coloured by chain, side view.
PDB entry 2xic coloured by chain, top view.
Source organism: Streptococcus pyogenes
Primary publication:
A highly unusual thioester bond in a pilus adhesin is required for efficient host cell interaction.
Pointon JA, Smith WD, Saalbach G, Crow A, Kehoe MA, Banfield MJ
J Biol Chem 285 33858-66 (2010)
PMID: 20729215

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184560 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ancillary protein 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 457 amino acids
Theoretical weight: 51.48 KDa
Source organism: Streptococcus pyogenes
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8GRA2 (Residues: 286-723; Coverage: 58%)
Gene name: cpa1
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P212121
Unit cell:
a: 43.1Å b: 113.32Å c: 175.36Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.233 0.231 0.274
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

12 review citations

Architects at the bacterial surface - sortases and the assembly of pili with isopeptide bonds.
Hendrickx et al. (2011)
11 more

6 mentions without citation

Pilus biogenesis of Gram-positive bacteria: Roles of sortases and implications for assembly.
Khare et al. (2017)
5 more