› 2xhm

X-ray diffraction
1.96Å resolution

Crystal structure of AnCE-K26 complex

Released:
DOI: 10.2210/pdb2xhm/pdb
PDB entry 2xhm coloured by chain, front view.
PDB entry 2xhm coloured by chain, side view.
PDB entry 2xhm coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Crystal structure of a phosphonotripeptide K-26 in complex with angiotensin converting enzyme homologue (AnCE) from Drosophila melanogaster.
Akif M, Ntai I, Sturrock ED, Isaac RE, Bachmann BO, Acharya KR
Biochem Biophys Res Commun 398 532-6 (2010)
PMID: 20599761

Function and Biology Details

Reaction catalysed: 
Release of a C-terminal dipeptide, oligopeptide-|-Xaa-Yaa, when Xaa is not Pro, and Yaa is neither Asp nor Glu. Thus, conversion of angiotensin I to angiotensin II, with increase in vasoconstrictor activity, but no action on angiotensin II.
Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171246 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Angiotensin-converting enzyme Chain: A
Molecule details ›
Chain: A
Length: 598 amino acids
Theoretical weight: 69.15 KDa
Source organism: Drosophila melanogaster
Expression system: Komagataella phaffii GS115
UniProt:
  • Canonical: Q10714 (Residues: 17-614; Coverage: 100%)
Gene names: Ance, CG8827, Race
Sequence domains: Angiotensin-converting enzyme

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
4 bound ligands:
Ligand K26 1 x K26
Ligand ZN 1 x ZN
Ligand EPE 1 x EPE
Ligand NAG 2 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: R3
Unit cell:
a: 172.707Å b: 172.707Å c: 101.696Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.195 0.194 0.215
Expression system: Komagataella phaffii GS115

Quick links

Citations

4 review citations

Different in vivo functions of the two catalytic domains of angiotensin-converting enzyme (ACE).
Bernstein et al. (2011)
3 more

1 mention without citation

Crystal structure of a peptidyl-dipeptidase K-26-DCP from Actinomycete in complex with its natural inhibitor.
Masuyer et al. (2016)