› 2xha

X-ray diffraction
1.91Å resolution

Crystal Structure of Domain 2 of Thermotoga maritima N-utilization Substance G (NusG)

Released:
DOI: 10.2210/pdb2xha/pdb
PDB entry 2xha coloured by chain, front view.
PDB entry 2xha coloured by chain, side view.
PDB entry 2xha coloured by chain, top view.
Source organism: Thermotoga maritima
Primary publication:
An autoinhibited state in the structure of Thermotoga maritima NusG.
Drögemüller J, Stegmann CM, Mandal A, Steiner T, Burmann BM, Gottesman ME, Wöhrl BM, Rösch P, Wahl MC, Schweimer K
Structure 21 365-75 (2013)
PMID: 23415559

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151460 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcription termination/antitermination protein NusG Chains: A, B
Molecule details ›
Chains: A, B
Length: 193 amino acids
Theoretical weight: 22.12 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P29397 (Residues: 41-233; Coverage: 55%)
Gene names: TM_0453, nusG
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand ACT 7 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MPG/DESY, HAMBURG BEAMLINE BW6
Spacegroup: C2
Unit cell:
a: 136.35Å b: 42.264Å c: 88.36Å
α: 90° β: 106.56° γ: 90°
R-values:
R R work R free
0.184 0.181 0.23
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Ubiquitous transcription factors display structural plasticity and diverse functions: NusG proteins - Shifting shapes and paradigms.
NandyMazumdar et al. (2015)
1 more

24 mentions without citation

Endogenous DNA 3' Blocks Are Vulnerabilities for BRCA1 and BRCA2 Deficiency and Are Reversed by the APE2 Nuclease.
Álvarez-Quilón et al. (2020)
23 more