› 2xh2

X-ray diffraction
1.8Å resolution

Engineering the enolase active site pocket: Crystal structure of the S39N D321A mutant of yeast enolase 1

Released:
DOI: 10.2210/pdb2xh2/pdb
PDB entry 2xh2 coloured by chain, front view.
PDB entry 2xh2 coloured by chain, side view.
PDB entry 2xh2 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Engineering the enolase magnesium II binding site: implications for its evolution.
Schreier B, Höcker B
Biochemistry 49 7582-9 (2010)
PMID: 20690637

Function and Biology Details

Reaction catalysed: 
2-phospho-D-glycerate = phosphoenolpyruvate + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133690 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Enolase 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 443 amino acids
Theoretical weight: 47.67 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P00924 (Residues: 2-437; Coverage: 100%)
Gene names: ENO1, ENOA, G9160, HSP48, YGR254W
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 4 x MG
Ligand 2PG 4 x 2PG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P1
Unit cell:
a: 64.72Å b: 82.54Å c: 95.61Å
α: 89.41° β: 71.31° γ: 84.8°
R-values:
R R work R free
0.168 0.166 0.209
Expression system: Escherichia coli BL21

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Citations

1 review citation

Biochemical functional predictions for protein structures of unknown or uncertain function.
Mills et al. (2015)
13 other articles