› 2xew

X-ray diffraction
2.2Å resolution

Crystal structure of K11-linked diubiquitin

Released:
DOI: 10.2210/pdb2xew/pdb
PDB entry 2xew coloured by chain, front view.
PDB entry 2xew coloured by chain, side view.
PDB entry 2xew coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Lys11-linked ubiquitin chains adopt compact conformations and are preferentially hydrolyzed by the deubiquitinase Cezanne.
Bremm A, Freund SM, Komander D
Nat Struct Mol Biol 17 939-47 (2010)
PMID: 20622874

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143449 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 76 amino acids
Theoretical weight: 8.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P0CG48 (Residues: 609-684; Coverage: 11%)
Gene name: UBC
Sequence domains: Ubiquitin family
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1

Ligands and Environments

3 bound ligands:
Ligand EDO 17 x EDO
Ligand CL 7 x CL
Ligand FLC 2 x FLC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P2221
Unit cell:
a: 79.23Å b: 79.96Å c: 221.23Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.205 0.252
Expression system: Escherichia coli

Quick links

Citations

61 review citations

The ubiquitin code.
Komander et al. (2012)
60 more

17 mentions without citation

K11-linked ubiquitin chains as novel regulators of cell division.
Wickliffe et al. (2011)
16 more