› 2xe4

X-ray diffraction
1.65Å resolution

Structure of Oligopeptidase B from Leishmania major

Released:
DOI: 10.2210/pdb2xe4/pdb
PDB entry 2xe4 coloured by chain, front view.
PDB entry 2xe4 coloured by chain, side view.
PDB entry 2xe4 coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structure of Leishmania major oligopeptidase B gives insight into the enzymatic properties of a trypanosomatid virulence factor.
McLuskey K, Paterson NG, Bland ND, Isaacs NW, Mottram JC
J Biol Chem 285 39249-59 (2010)
PMID: 20926390

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-163315 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Prolyl endopeptidase Chain: A
Molecule details ›
Chain: A
Length: 751 amino acids
Theoretical weight: 85.24 KDa
Source organism: Leishmania major
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q4QHU7 (Residues: 1-731; Coverage: 100%)
Gene names: LMJF_09_0770, OPB
Sequence domains:
Structure domains:
ANTIPAIN Chain: B
Molecule details ›
Chain: B
Length: 4 amino acids
Theoretical weight: 607 Da
Source organism: Actinomycetes

Ligands and Environments

6 bound ligands:
Ligand GOL 10 x GOL
Ligand PGR 48 x PGR
Ligand PGO 1 x PGO
Ligand PO4 3 x PO4
Ligand CL 4 x CL
Ligand NA 2 x NA
2 modified residues:
Ligand FC0 1 x FC0
Ligand RGL 1 x RGL

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007, ESRF BEAMLINE BM14
Spacegroup: I222
Unit cell:
a: 95.478Å b: 142.776Å c: 208.919Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.142 0.14 0.178
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

21 citation in other articles

Oligopeptidase B deficient mutants of Leishmania major.
Munday et al. (2011)
20 more

13 mentions without citation

Copaifera of the Neotropics: A Review of the Phytochemistry and Pharmacology.
da Trindade et al. (2018)
12 more