2xbm

X-ray diffraction
2.9Å resolution

Crystal structure of the dengue virus methyltransferase bound to a 5'- capped octameric RNA

Released:

Function and Biology Details

Reactions catalysed:
Selective hydrolysis of -Xaa-Xaa-|-Yaa- bonds in which each of the Xaa can be either Arg or Lys and Yaa can be either Ser or Ala.
NTP + H(2)O = NDP + phosphate
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-111012 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
NONSTRUCTURAL PROTEIN NS5 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 263 amino acids
Theoretical weight: 29.8 KDa
Source organism: Dengue virus
UniProt:
  • Canonical: C0LMU5 (Residues: 2491-2753; Coverage: 8%)
Sequence domains: FtsJ-like methyltransferase
Structure domains: Vaccinia Virus protein VP39
5'-(*G3AP*GP*AP*AP*CP*CP*UP*GP*A)-3' Chains: E, F
Molecule details ›
Chains: E, F
Length: 9 nucleotides
Theoretical weight: 3.3 KDa
Source organism: Dengue virus
Expression system: Not provided

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P32
Unit cell:
a: 137.543Å b: 137.543Å c: 109.385Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.205 0.204 0.231
Expression system: Not provided