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80 microsecond Laue diffraction snapshot from crystals of a photosynthetic reaction centre without illumination.

X-ray diffraction
3Å resolution
PDB entry 2x5u coloured by chain, front view.
PDB entry 2x5u coloured by chain, side view.
PDB entry 2x5u coloured by chain, top view.
Source organism: Blastochloris viridis
Assembly composition:
hetero tetramer (preferred)
Entry contents: 4 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero tetramer
Accessible surface area: 42493.84 Å2
Buried surface area: 44261.25 Å2
Dissociation area: 4,805.6 Å2
Dissociation energy (ΔGdiss): 53.91 kcal/mol
Dissociation entropy (TΔSdiss): 15.13 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-138851

Macromolecules

Photosynthetic reaction center cytochrome c subunit
Chain: C
Length: 336 amino acids
Theoretical weight: 37.45 KDa
Source organism: Blastochloris viridis
UniProt:
  • Canonical: P07173 (Residues: 21-356; Coverage: 100%)
Gene names: BVIRIDIS_00500, cytC, pufC
Pfam: Photosynthetic reaction centre cytochrome C subunit
InterPro:
CATH: Photosynthetic Reaction Center, subunit C, domain 2
Photosynthetic reaction center cytochrome c subunit in PDB entry 2x5u, assembly 1, front view.
Photosynthetic reaction center cytochrome c subunit in PDB entry 2x5u, assembly 1, side view.
Photosynthetic reaction center cytochrome c subunit in PDB entry 2x5u, assembly 1, top view.
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Reaction center protein H chain
Chain: H
Length: 258 amino acids
Theoretical weight: 28.56 KDa
Source organism: Blastochloris viridis
UniProt:
  • Canonical: P06008 (Residues: 1-258; Coverage: 100%)
Gene name: puhA
Pfam:
InterPro:
CATH:
Reaction center protein H chain in PDB entry 2x5u, assembly 1, front view.
Reaction center protein H chain in PDB entry 2x5u, assembly 1, side view.
Reaction center protein H chain in PDB entry 2x5u, assembly 1, top view.
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Reaction center protein L chain
Chain: L
Length: 274 amino acids
Theoretical weight: 30.6 KDa
Source organism: Blastochloris viridis
UniProt:
  • Canonical: P06009 (Residues: 1-274; Coverage: 100%)
Gene name: pufL
Pfam: Photosynthetic reaction centre protein
InterPro:
CATH: Photosystem II protein D1-like
Reaction center protein L chain in PDB entry 2x5u, assembly 1, front view.
Reaction center protein L chain in PDB entry 2x5u, assembly 1, side view.
Reaction center protein L chain in PDB entry 2x5u, assembly 1, top view.
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Reaction center protein M chain
Chain: M
Length: 324 amino acids
Theoretical weight: 36.06 KDa
Source organism: Blastochloris viridis
UniProt:
  • Canonical: P06010 (Residues: 1-324; Coverage: 100%)
Gene name: pufM
Pfam: Photosynthetic reaction centre protein
InterPro:
CATH: Photosystem II protein D1-like
Reaction center protein M chain in PDB entry 2x5u, assembly 1, front view.
Reaction center protein M chain in PDB entry 2x5u, assembly 1, side view.
Reaction center protein M chain in PDB entry 2x5u, assembly 1, top view.
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