› 2x42

X-ray diffraction
2.1Å resolution

Structure of beta-glucosidase 3B from Thermotoga neapolitana in complex with alpha-D-glucose

Released:
DOI: 10.2210/pdb2x42/pdb
PDB entry 2x42 coloured by chain, front view.
PDB entry 2x42 coloured by chain, side view.
PDB entry 2x42 coloured by chain, top view.
Source organism: Thermotoga neapolitana DSM 4359
Primary publication:
Structural and functional analyses of beta-glucosidase 3B from Thermotoga neapolitana: a thermostable three-domain representative of glycoside hydrolase 3.
Pozzo T, Pasten JL, Karlsson EN, Logan DT
J Mol Biol 397 724-39 (2010)
PMID: 20138890

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170928 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fibronectin type III-like domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 721 amino acids
Theoretical weight: 81.22 KDa
Source organism: Thermotoga neapolitana DSM 4359
Expression system: Escherichia coli
UniProt:
  • Canonical: Q0GC07 (Residues: 1-721; Coverage: 100%)
Gene name: bgl3B
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand GLC 1 x GLC
Ligand BR 4 x BR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-2
Spacegroup: C2221
Unit cell:
a: 74.55Å b: 128.16Å c: 175.65Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.172 0.214
Expression system: Escherichia coli

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Citations

4 review citations

Biotransformation of ginsenosides by hydrolyzing the sugar moieties of ginsenosides using microbial glycosidases.
Park et al. (2010)
3 more

4 mentions without citation

Development of cellobiose-degrading ability in Yarrowia lipolytica strain by overexpression of endogenous genes.
Guo et al. (2015)
3 more